Identification of multiple SRF N-terminal phosphorylation sites affecting DNA binding properties.

Human serum response factor (SRF) bearing a histidine tag was expressed using vaccinia virus. The recombinant protein was purified and shown to be phosphorylated mainly in its N-terminal part. The corresponding phosphorylation sites were mapped by microsequencing and also appear to be phosphorylated...

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Main Authors: Janknecht, R, Hipskind, R A, Houthaeve, T, Nordheim, A, Stunnenberg, H G
Format: Artigo
Sprog:Inglês
Udgivet: 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC556545/
https://ncbi.nlm.nih.gov/pubmed/1547771
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