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Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Cells constantly change their membrane architecture and protein distribution, but it is extremely difficult to visualize these events at a temporal and spatial resolution on the order of ms and nm, respectively. We have developed a time-resolved electron microscopy technique, "flash-and-freeze,...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Li, Shuo, Raychaudhuri, Sumana, Watanabe, Shigeki
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5565139/
https://ncbi.nlm.nih.gov/pubmed/28518090
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55664
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