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Identification and characterization of yeast mutants and the gene for a cruciform cutting endonuclease.

An assay was developed that detected DNA cruciform cutting endonuclease activity in crude extracts of Saccharomyces cerevisiae. A collection of temperature-sensitive strains was screened using this assay, and a mutant lacking the activity was found. The mutation leading to the enzymatic defect was m...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Kleff, S, Kemper, B, Sternglanz, R
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1992
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC556502/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1537343/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1992.tb05102.x
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