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Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy
Live-cell imaging is used to simultaneously capture time-lapse images of angiotensin type 1a receptors (AT(1a)R) and intracellular compartments in transfected human embryonic kidney-293 (HEK) cells following stimulation with angiotensin II (Ang II). HEK cells are transiently transfected with plasmid...
Guardat en:
| Publicat a: | J Vis Exp |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MyJove Corporation
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5564435/ https://ncbi.nlm.nih.gov/pubmed/28447987 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55177 |
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