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Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen

We present a novel method for shockwave-assisted bacterial transformation using a miniature oxyhydrogen detonation-driven shock tube. We have obtained transformation efficiencies of about 1.28 × 10(6), 1.7 × 10(6), 5 × 10(6), 1 × 10(5), 1 × 10(5) and 2 × 10(5) transformants/µg of DNA for Escherichia...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Datey, Akshay, Subburaj, Janardhanraj, Gopalan, Jagadeesh, Chakravortty, Dipshikha
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5561244/
https://ncbi.nlm.nih.gov/pubmed/28819202
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-08542-5
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