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Evaluation of reference genes for reverse transcription quantitative real-time PCR (RT-qPCR) studies in Silene vulgaris considering the method of cDNA preparation

Accurate gene expression measurements are essential in studies of both crop and wild plants. Reverse transcription quantitative real-time PCR (RT-qPCR) has become a preferred tool for gene expression estimation. A selection of suitable reference genes for the normalization of transcript levels is an...

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Détails bibliographiques
Publié dans:PLoS One
Auteurs principaux: Koloušková, Pavla, Stone, James D., Štorchová, Helena
Format: Artigo
Langue:Inglês
Publié: Public Library of Science 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5560574/
https://ncbi.nlm.nih.gov/pubmed/28817728
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183470
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