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Ex vivo tools for the clonal analysis of zebrafish hematopoiesis
This protocol describes the ex vivo characterization of zebrafish hematopoietic progenitors. We show how to isolate zebrafish hematopoietic cells for cultivation and differentiation in colony assays in semi-solid media. We also describe procedures for the generation of recombinant zebrafish cytokine...
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Publicado no: | Nat Protoc |
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Main Authors: | , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5560128/ https://ncbi.nlm.nih.gov/pubmed/27123951 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.053 |
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