Ex vivo tools for the clonal analysis of zebrafish hematopoiesis

This protocol describes the ex vivo characterization of zebrafish hematopoietic progenitors. We show how to isolate zebrafish hematopoietic cells for cultivation and differentiation in colony assays in semi-solid media. We also describe procedures for the generation of recombinant zebrafish cytokine...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Svoboda, Ondrej, Stachura, David L, Machonova, Olga, Zon, Leonard I, Traver, David, Bartunek, Petr
Format: Artigo
Sprog:Inglês
Udgivet: 2016
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5560128/
https://ncbi.nlm.nih.gov/pubmed/27123951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.053
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