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Ex vivo tools for the clonal analysis of zebrafish hematopoiesis

This protocol describes the ex vivo characterization of zebrafish hematopoietic progenitors. We show how to isolate zebrafish hematopoietic cells for cultivation and differentiation in colony assays in semi-solid media. We also describe procedures for the generation of recombinant zebrafish cytokine...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Nat Protoc
Main Authors: Svoboda, Ondrej, Stachura, David L, Machonova, Olga, Zon, Leonard I, Traver, David, Bartunek, Petr
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5560128/
https://ncbi.nlm.nih.gov/pubmed/27123951
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2016.053
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