Construction of novel pJRD215-derived plasmids using chloramphenicol acetyltransferase (cat) gene as a selection marker for Acidithiobacillus caldus

BACKGROUND: Acidithiobacillus caldus, a Gram-negative, chemolithotrophic sulfur-oxidizing bacterium, is widely applied in bioleaching. The absence of an ideal selection marker has become a major obstacle to achieve high efficiency of the gene transfer system for A. caldus. Plasmid pJRD215, widely us...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Wang, Rui, Lin, Chunmao, Lin, Jianqiang, Pang, Xin, Liu, Xiangmei, Zhang, Chengjia, Lin, Jianqun, Chen, Linxu
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5559103/
https://ncbi.nlm.nih.gov/pubmed/28813510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183307
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