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Protease cleavage site fingerprinting by label‐free in‐gel degradomics reveals pH‐dependent specificity switch of legumain

Determination of protease specificity is of crucial importance for understanding protease function. We have developed the first gel‐based label‐free proteomic approach (DIPPS—direct in‐gel profiling of protease specificity) that enables quick and reliable determination of protease cleavage specifici...

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Foilsithe in:EMBO J
Main Authors: Vidmar, Robert, Vizovišek, Matej, Turk, Dušan, Turk, Boris, Fonović, Marko
Formáid: Artigo
Teanga:Inglês
Foilsithe: John Wiley and Sons Inc. 2017
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Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5556264/
https://ncbi.nlm.nih.gov/pubmed/28733325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201796750
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