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Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette

Gene manipulation is essential for metabolic engineering and synthetic biology, but the current general gene manipulation methods are not applicable to the non-model strain Corynebacterium glutamicum (C. glutamicum) ATCC14067, which is used for amino acid production. Here, we report an effective and...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Huang, Yuanyuan, Li, Lu, Xie, Shan, Zhao, Nannan, Han, Shuangyan, Lin, Ying, Zheng, Suiping
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5554157/
https://ncbi.nlm.nih.gov/pubmed/28801604
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-08352-9
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