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A transformation system for the hypotrichous ciliate Stylonychia mytilus

We describe a transformation system for the ciliate Stylonychia mytilus. The neomycin resistance gene from Escherichia coli transposon Tn5, which codes for the enzyme phosphotransferase and confers resistance to the antibiotic G 418, was ligated into macronuclear `gene-size' DNA molecules. Usin...

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Main Authors: Wünning, Ingrid U., Lipps, Hans J.
Format: Artigo
Jezik:Inglês
Izdano: 1983
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC555354/
https://ncbi.nlm.nih.gov/pubmed/16453475
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