Purification of snRNPs U1, U2, U4, U5 and U6 with 2,2,7-trimethylguanosine-specific antibody and definition of their constituent proteins reacting with anti-Sm and anti-(U1)RNP antisera.

Small nuclear ribonucleoprotein particles (snRNPs) of the U-snRNP class from Ehrlich ascites tumor cells were purified in a one-step procedure by affinity chromatography with antibodies specific for 2,2,7-trimethylguanosine (m23.2.7G), which is part of the 5'-terminal cap structure of snRNAs U1...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Bringmann, P, Rinke, J, Appel, B, Reuter, R, Lührmann, R
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1983
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC555246/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6194992/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1983.tb01557.x
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