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A dual-color reporter assay of cohesin-mediated gene regulation in budding yeast meiosis
In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes...
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| Publicat a: | Methods Mol Biol |
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| Autors principals: | , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5551346/ https://ncbi.nlm.nih.gov/pubmed/27797078 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6545-8_9 |
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