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A dual-color reporter assay of cohesin-mediated gene regulation in budding yeast meiosis

In this chapter, we describe a quantitative fluorescence-based assay of gene expression using the ratio of the reporter green fluorescence protein (GFP) to the internal red fluorescence protein (RFP) control. With this dual-color heterologous reporter assay, we have revealed cohesin-regulated genes...

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Dettagli Bibliografici
Pubblicato in:Methods Mol Biol
Autori principali: Fan, Jinbo, Jin, Hui, Yu, Hong-Guo
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2017
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5551346/
https://ncbi.nlm.nih.gov/pubmed/27797078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-6545-8_9
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