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Dynamics of cruciform extrusion in supercoiled DNA: use of a synthetic inverted repeat to study conformational populations.

An inverted repeat has been created in a plasmid by ligation of two 13 nucleotide synthetic oligonucleotides into the cloning vector pAT153. The resulting recombinant plasmid, pIRbke8, is hypersensitive to cleavage by the single-strand-specific nuclease S1, and to modification by the single-strand-s...

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Detalhes bibliográficos
Publicado no:EMBO J
Principais autores: Lilley, D M, Markham, A F
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC555056/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6628359/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1983.tb01458.x
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