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Dynamics of cruciform extrusion in supercoiled DNA: use of a synthetic inverted repeat to study conformational populations.
An inverted repeat has been created in a plasmid by ligation of two 13 nucleotide synthetic oligonucleotides into the cloning vector pAT153. The resulting recombinant plasmid, pIRbke8, is hypersensitive to cleavage by the single-strand-specific nuclease S1, and to modification by the single-strand-s...
Enregistré dans:
| Publié dans: | EMBO J |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Nature Publishing Group
1983
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC555056/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6628359/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1983.tb01458.x |
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