NMR Characterization of HtpG, the E. coli Hsp90, Using Sparse Labeling with (13)C-Methyl Alanine

A strategy for acquiring structural information from sparsely isotopically labeled large proteins is illustrated with an application to the E. coli heat-shock protein, HtpG (high temperature protein G), a 145 kDa dimer. It uses (13)C-alanine methyl labeling in a perdeuterated background to take adva...

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Bibliographic Details
Published in:J Biomol NMR
Main Authors: Pederson, Kari, Chalmers, Gordon R., Gao, Qi, Elnatan, Daniel, Ramelot, Theresa A., Ma, Li-Chung, Montelione, Gaetano T., Kennedy, Michael A., Agard, David A., Prestegard, James H.
Format: Artigo
Language:Inglês
Published: 2017
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5546222/
https://ncbi.nlm.nih.gov/pubmed/28653216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-017-0123-8
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