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Chlamydia trachomatis transformation and allelic exchange mutagenesis

Gene inactivation is essential for forward and reverse genetic approaches to establish protein function. Techniques such as insertion or chemical mutagenesis have been developed to mutagenize chlamydiae via targeted or random mutagenesis, respectively. Both of these approaches require transformation...

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Bibliographic Details
Published in:Curr Protoc Microbiol
Main Authors: Mueller, Konrad E., Wolf, Katerina, Fields, Kenneth A.
Format: Artigo
Language:Inglês
Published: 2017
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5545879/
https://ncbi.nlm.nih.gov/pubmed/28510361
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmc.31
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