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Analytical sensitivity and specificity of a loop-mediated isothermal amplification (LAMP) kit prototype for detection of Trypanosoma cruzi DNA in human blood samples

This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection of Trypanosoma cruzi DNA in human blood. The prototype is based on the amplification of the highly repetitive satellite sequence of T.cruzi in microtubes containing dried reagents on the inside o...

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Detalhes bibliográficos
Publicado no:PLoS Negl Trop Dis
Main Authors: Besuschio, Susana A., Llano Murcia, Mónica, Benatar, Alejandro F., Monnerat, Severine, Cruz, Israel, Picado, Albert, Curto, María de los Ángeles, Kubota, Yutaka, Wehrendt, Diana P., Pavia, Paula, Mori, Yasuyoshi, Puerta, Concepción, Ndung'u, Joseph M., Schijman, Alejandro G.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5544240/
https://ncbi.nlm.nih.gov/pubmed/28727723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pntd.0005779
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