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Initiation, elongation and pausing of in vitro DNA synthesis catalyzed by immunopurified yeast DNA primase: DNA polymerase complex.

Yeast DNA primase and DNA polymerase I can be purified by immunoaffinity chromatography as a multipeptide complex which can then be resolved into its functional components and further reassembled in vitro. Isolated DNA primase synthesizes oligonucleotides of a preferred length of 9-10 nucleotides an...

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Publicat a:EMBO J
Autors principals: Badaracco, G, Bianchi, M, Valsasnini, P, Magni, G, Plevani, P
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 1985
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC554342/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4006921/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1985.tb03778.x
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