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High-Resolution Optical Tweezers Combined With Single-Molecule Confocal Microscopy

We describe the design, construction, and application of an instrument combining dual-trap, high-resolution optical tweezers and a confocal microscope. This hybrid instrument allows nanomechanical manipulation and measurement simultaneously with single-molecule fluorescence detection. We present the...

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Detalles Bibliográficos
Publicado en:Methods Enzymol
Main Authors: Whitley, K.D., Comstock, M.J., Chemla, Y.R.
Formato: Artigo
Idioma:Inglês
Publicado: 2016
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5540136/
https://ncbi.nlm.nih.gov/pubmed/28062033
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mie.2016.10.036
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