Construction and use of chimeric SPR/phi 3T DNA methyltransferases in the definition of sequence recognizing enzyme regions.

Multispecific DNA methyltransferases (Mtases) of temperate Bacillus subtilis phages SPR and phi 3T methylate the internal cytosine of the sequence GGCC. They differ in their capacity to methylate additional sequences. These are CCGG and CC(A/T)GG in SPR and GCNGC in phi 3T. Introducing unique restri...

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Main Authors: Balganesh, T S, Reiners, L, Lauster, R, Noyer-Weidner, M, Wilke, K, Trautner, T A
Format: Artigo
Jezik:Inglês
Izdano: 1987
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC553815/
https://ncbi.nlm.nih.gov/pubmed/2828032
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