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In vitro assembly of U1 snRNPs.

An efficient system for the in vitro assembly of U1 snRNPs is described. RNA-protein interactions in a series of U1 snRNA mutants assembled both in vivo and in vitro were studied in order to verify the accuracy of the system. Two discrete protein binding sites are defined by immunoprecipitation with...

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Bibliographic Details
Main Authors: Hamm, J, Kazmaier, M, Mattaj, I W
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC553806/
https://ncbi.nlm.nih.gov/pubmed/2962858
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