Extreme assay sensitivity in molecular diagnostics further unveils intratumour heterogeneity in metastatic colorectal cancer as well as artifactual low-frequency mutations in the KRAS gene

BACKGROUND: Gene mutations in the RAS family rule out metastatic colorectal carcinomas (mCRCs) from anti-EGFR therapies. METHODS: We report a retrospective analysis by Sequenom Massarray and fast COLD-PCR followed by Sanger sequencing on 240 mCRCs. RESULTS: By Sequenom, KRAS and NRAS exons 2-3-4 wer...

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Vydáno v:Br J Cancer
Hlavní autoři: Mariani, Sara, Bertero, Luca, Osella-Abate, Simona, Di Bello, Cristiana, Francia di Celle, Paola, Coppola, Vittoria, Sapino, Anna, Cassoni, Paola, Marchiò, Caterina
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group 2017
Témata:
Molecular Diagnostics
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5537488/
https://ncbi.nlm.nih.gov/pubmed/28618430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/bjc.2017.170
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