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Effects of DNA sequence and structure on binding of RecA to single-stranded DNA
Fluorescence anisotropy is used to follow the binding of RecA to short single-stranded DNA (ssDNA) sequences (39 bases) at low DNA and RecA concentration where the initial phase of polymerization occurs. We observe that RecA condensation is extremely sensitive to minute changes in DNA sequences. Rec...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The National Academy of Sciences
2001
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC55374/ https://ncbi.nlm.nih.gov/pubmed/11470894 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.151242898 |
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