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Highly sensitive protein detection by biospecific AFM‐based fishing with pulsed electrical stimulation

We report here the highly sensitive detection of protein in solution at concentrations from 10(–15) to 10(–18) m using the combination of atomic force microscopy (AFM) and mass spectrometry. Biospecific detection of biotinylated bovine serum albumin was carried out by fishing out the protein onto th...

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Bibliographische Detailangaben
Veröffentlicht in:FEBS Open Bio
Hauptverfasser: Pleshakova, Tatyana O., Malsagova, Kristina A., Kaysheva, Anna L., Kopylov, Arthur T., Tatur, Vadim Yu., Ziborov, Vadim S., Kanashenko, Sergey L., Galiullin, Rafael A., Ivanov, Yuri D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: John Wiley and Sons Inc. 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5537060/
https://ncbi.nlm.nih.gov/pubmed/28781958
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/2211-5463.12253
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