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A novel method for site-directed mutagenesis: its application to an eukaryotic tRNAPro gene promoter.

We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which re...

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Main Authors: Traboni, C, Ciliberto, G, Cortese, R
Format: Artigo
Jezik:Inglês
Izdano: 1982
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC553061/
https://ncbi.nlm.nih.gov/pubmed/6329678
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