Homologous recombination at c-fyn locus of mouse embryonic stem cells with use of diphtheria toxin A-fragment gene in negative selection.
In attempting to produce a mutant mouse with embryonic stem cells, the critical step is the efficient isolation of homologous recombinants; the frequency of the homologous recombination is usually low and the potency of the cells to differentiate into germ cells is unstable in culture. Here, we repo...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1990
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC55285/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2263643/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.24.9918 |
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