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Isolation and Infection of Drosophila Primary Hemocytes

Phagocytosis of invading pathogens and their subsequent clearance in lysosomes is important for organismal fitness. We have devised the following protocol to extract phagocytic hemocytes from wild-type and mutant Drosophila larvae and infect the isolated hemocytes with GFP-labeled E. coli to measure...

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Bibliographische Detailangaben
Veröffentlicht in:Bio Protoc
Hauptverfasser: Tracy, Charles, Krämer, Helmut
Format: Artigo
Sprache:Inglês
Veröffentlicht: Bio-Protocol 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5527747/
https://ncbi.nlm.nih.gov/pubmed/28758141
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2300
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