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Isolation and Infection of Drosophila Primary Hemocytes
Phagocytosis of invading pathogens and their subsequent clearance in lysosomes is important for organismal fitness. We have devised the following protocol to extract phagocytic hemocytes from wild-type and mutant Drosophila larvae and infect the isolated hemocytes with GFP-labeled E. coli to measure...
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| Published in: | Bio Protoc |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Bio-Protocol
2017
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5527747/ https://ncbi.nlm.nih.gov/pubmed/28758141 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2300 |
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