Molecular cloning, heterologous expression, and functional characterization of a cellulolytic enzyme (Cel PRII) from buffalo rumen metagenome

A cellulase encoding gene, Cel PRII, was identified from Mehsani buffalo rumen metagenome, and cloned and expressed in Escherichia coli BL21(DE3)pLysS. The 1170 bp full length gene encodes a 389 residue polypeptide (Cel PRII) containing a catalytic domain belonging to glycosyl hydrolase (GH) 5 famil...

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Publicado en:3 Biotech
Main Authors: Shah, Ravi K., Patel, Amrutlal K., Davla, Deepti M., Parikh, Ishan K., Subramanian, Ramalingam B., Patel, Kamlesh C., Jakhesara, Subhash J., Joshi, Chaitanya G.
Formato: Artigo
Idioma:Inglês
Publicado: Springer Berlin Heidelberg 2017
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Original Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5520813/
https://ncbi.nlm.nih.gov/pubmed/28733938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-017-0895-2
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