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Mutational analysis of ligand binding activity of beta 2 adrenergic receptor expressed in Escherichia coli.

A novel in situ screening procedure was used to identify neutral mutations in the human beta 2 adrenergic receptor (beta 2AR). The coding region of the human beta 2AR gene was subcloned under transcriptional control of an inducible T7 promoter and used to transform Escherichia coli. Colonies express...

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Enregistré dans:
Détails bibliographiques
Publié dans:EMBO J
Auteurs principaux: Breyer, R M, Strosberg, A D, Guillet, J G
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group 1990
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC551972/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2167830/
https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1990.tb07453.x
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