Purification and Biological Characterization of Shiga Toxin from Shigella dysenteriae 1

Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, and...

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Main Authors: Brown, J. Edward, Griffin, Darrell E., Rothman, Sara W., Doctor, B. P.
Format: Artigo
Jezik:Inglês
Izdano: 1982
Teme:
Pathogenic Mechanisms
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC551430/
https://ncbi.nlm.nih.gov/pubmed/7047403
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