Purification and Biological Characterization of Shiga Toxin from Shigella dysenteriae 1

Shiga toxin has been purified in milligram quantities to near homogeneity from cell lysates of Shigella dysenteriae 1 strain 3818-0. Purification involved an initial ultracentrifugation, ammonium sulfate fractionation, chromatography on DEAE-cellulose and carboxymethyl cellulose, gel filtration, and...

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Detalhes bibliográficos
Publicado no:Infect Immun
Main Authors: Brown, J. Edward, Griffin, Darrell E., Rothman, Sara W., Doctor, B. P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1982
Assuntos:
Pathogenic Mechanisms
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC551430/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7047403/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.36.3.996-1005.1982
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