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Pitfalls in genetic testing: a case of a SNP in primer‐annealing region leading to allele dropout in BRCA1

BACKGROUND: Hereditary breast and ovarian cancer is characterized by mutations in BRCA1 or BRCA2 genes and PCR‐based screening techniques, such as capillary sequencing and next‐generation sequencing (NGS), are considered gold standard methods for detection of pathogenic mutations in these genes. Sin...

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Detalhes bibliográficos
Publicado no:Mol Genet Genomic Med
Main Authors: Silva, Felipe Carneiro, Torrezan, Giovana Tardin, Brianese, Rafael Canfield, Stabellini, Raquel, Carraro, Dirce Maria
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5511807/
https://ncbi.nlm.nih.gov/pubmed/28717669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/mgg3.295
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