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FTIR spectroscopy of whole cells for the monitoring of yeast apoptosis mediated by p53 over-expression and its suppression by Nigella sativa extracts

p53 over expression in yeast results in cell death with typical markers of apoptosis such as DNA fragmentation and phosphatidylserine externalization. We aimed to substitute/supplement classical fluorescent techniques (TUNEL, Annexin V, ROS detection) usually used to detect biochemical changes occur...

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Publicat a:PLoS One
Autors principals: Mihoubi, Wafa, Sahli, Emna, Gargouri, Ali, Amiel, Caroline
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2017
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5507515/
https://ncbi.nlm.nih.gov/pubmed/28704406
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0180680
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