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Quantification of lactate dehydrogenase for cell viability testing using cell lines and primary cultured astrocytes

Drug discovery heavily relies on cell viability studies to assess the potential toxicity of drug candidates. L-Lactate dehydrogenase (LDH) is a cytoplasmic enzyme that catalyzes the concomitant interconversions of pyruvate to L-lactate and NADH to NAD(+) during glycolysis, and the reverse reactions...

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Detalhes bibliográficos
Publicado no:Curr Protoc Toxicol
Main Authors: Kaja, Simon, Payne, Andrew J., Naumchuk, Yuliya, Koulen, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5501254/
https://ncbi.nlm.nih.gov/pubmed/28463416
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cptx.21
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