Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing

Our objective was to determine if whole genome amplification (WGA) provides suitable DNA for qPCR-based genotyping for human embryos. Single blastomeres (Day 3) or trophoblastic cells (Day 5) were isolated from 342 embryos for WGA. Comparative Genomic Hybridization determined embryo sex as well as T...

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Detalles Bibliográficos
Publicado en:Biomed Res Int
Main Authors: Schaeffer, Elizabeth, López-Bayghen, Bruno, Neumann, Adina, Porchia, Leonardo M., Camacho, Rafael, Garrido, Efraín, Gómez, Rocío, Camargo, Felipe, López-Bayghen, Esther
Formato: Artigo
Idioma:Inglês
Publicado: Hindawi 2017
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5498896/
https://ncbi.nlm.nih.gov/pubmed/28717645
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2017/1209158
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