A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production

A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using t...

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Bibliografiske detaljer
Udgivet i:Braz J Microbiol
Main Authors: Yang, Weichao, Han, Litao, Mandlaa, Mandlaa, Zhang, Haihong, Zhang, Zhongze, Xu, Hui
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2017
Fag:
Biotechnology and Industrial Microbiology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5498444/
https://ncbi.nlm.nih.gov/pubmed/28292630
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bjm.2017.02.002
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