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Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ-(32)P]ATP in RNA structural investigation

Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 φ2.5 promoter. Efficient fluorophore labeling of 5′ RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solu...

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Bibliografiske detaljer
Main Authors: Li, Na, Yu, Changjun, Huang, Faqing
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2005
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC549576/
https://ncbi.nlm.nih.gov/pubmed/15731330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni036
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