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Novel cyanine-AMP conjugates for efficient 5′ RNA fluorescent labeling by one-step transcription and replacement of [γ-(32)P]ATP in RNA structural investigation
Two novel fluorescent cyanine-AMP conjugates, F550/570 and F650/670, have been synthesized to serve as transcription initiators under the T7 φ2.5 promoter. Efficient fluorophore labeling of 5′ RNA is achieved in a single transcription step by including F550/570 and F650/670 in the transcription solu...
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| Udgivet i: | Nucleic Acids Res |
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| Main Authors: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2005
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC549576/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15731330/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gni036 |
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