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Effective transcriptome amplification for expression profiling on sense-oriented oligonucleotide microarrays

Gene expression analysis using microarrays of synthetic long oligonucleotides is limited in that it requires substantial amounts of RNA. To obtain these quantities from minute amounts of starting material, protocols were developed that linearly amplify mRNA by cDNA synthesis and in vitro transcripti...

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Detalhes bibliográficos
Main Authors: Schlingemann, Joerg, Thuerigen, Olaf, Ittrich, Carina, Toedt, Grischa, Kramer, Heidi, Hahn, Meinhard, Lichter, Peter
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC549428/
https://ncbi.nlm.nih.gov/pubmed/15718295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni029
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