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Effective transcriptome amplification for expression profiling on sense-oriented oligonucleotide microarrays

Gene expression analysis using microarrays of synthetic long oligonucleotides is limited in that it requires substantial amounts of RNA. To obtain these quantities from minute amounts of starting material, protocols were developed that linearly amplify mRNA by cDNA synthesis and in vitro transcripti...

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Hlavní autoři: Schlingemann, Joerg, Thuerigen, Olaf, Ittrich, Carina, Toedt, Grischa, Kramer, Heidi, Hahn, Meinhard, Lichter, Peter
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC549428/
https://ncbi.nlm.nih.gov/pubmed/15718295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni029
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