An Optimized Shotgun Strategy for the Rapid Generation of Comprehensive Human Proteomes

This study investigates the challenge of comprehensively cataloging the complete human proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics. We modify a classical two-dimensional high-resolution reversed-phase peptide fractionation scheme and optimize a protocol tha...

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Bibliografske podrobnosti
izdano v:Cell Syst
Main Authors: Bekker-Jensen, Dorte B., Kelstrup, Christian D., Batth, Tanveer S., Larsen, Sara C., Haldrup, Christa, Bramsen, Jesper B., Sørensen, Karina D., Høyer, Søren, Ørntoft, Torben F., Andersen, Claus L., Nielsen, Michael L., Olsen, Jesper V.
Format: Artigo
Jezik:Inglês
Izdano: Cell Press 2017
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC5493283/
https://ncbi.nlm.nih.gov/pubmed/28601559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cels.2017.05.009
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