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Development and Use of a Real-Time Quantitative PCR Method for Detecting and Quantifying Equol-Producing Bacteria in Human Faecal Samples and Slurry Cultures

This work introduces a novel real-time quantitative PCR (qPCR) protocol for detecting and quantifying equol-producing bacteria. To this end, two sets of primers targeting the dihydrodaidzein reductase (ddr) and tetrahydrodaidzein reductase (tdr) genes, which are involved in the synthesis of equol, w...

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Detalles Bibliográficos
Publicado en:Front Microbiol
Main Authors: Vázquez, Lucía, Guadamuro, Lucía, Giganto, Froilán, Mayo, Baltasar, Flórez, Ana B.
Formato: Artigo
Idioma:Inglês
Publicado: Frontiers Media S.A. 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5491606/
https://ncbi.nlm.nih.gov/pubmed/28713336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.01155
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