Tuning the transcription and translation of L-amino acid deaminase in Escherichia coli improves α-ketoisocaproate production from L-leucine

α-Ketoisocaproate (KIC) is used widely in the pharmaceutical and nutraceutical industries. In previous studies, we achieved a one-step biosynthesis of KIC from l-leucine, using an Escherichia coli whole-cell biocatalyst expressing an l-amino acid deaminase (l-AAD) from Proteus vulgaris. Herein, we r...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Song, Yang, Li, Jianghua, Shin, Hyun-dong, Liu, Long, Du, Guocheng, Chen, Jian
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5491005/
https://ncbi.nlm.nih.gov/pubmed/28662040
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0179229
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