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Click-PEGylation – A mobility shift approach to assess the redox state of cysteines in candidate proteins

The redox state of cysteine thiols is critical for protein function. Whereas cysteines play an important role in the maintenance of protein structure through the formation of internal disulfides, their nucleophilic thiol groups can become oxidatively modified in response to diverse redox challenges...

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Detalhes bibliográficos
Publicado no:Free Radic Biol Med
Main Authors: van Leeuwen, Lucie A.G., Hinchy, Elizabeth C., Murphy, Michael P., Robb, Ellen L., Cochemé, Helena M.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier Science 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5488967/
https://ncbi.nlm.nih.gov/pubmed/28366801
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2017.03.037
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