Recombinant Mouse Hepatitis Virus Strain A59 from Cloned, Full-Length cDNA Replicates to High Titers In Vitro and Is Fully Pathogenic In Vivo

Mouse hepatitis virus (MHV) is the prototype of group II coronaviruses and one of the most extensively studied coronaviruses. Here, we describe a reverse genetic system for MHV (strain A59) based upon the cloning of a full-length genomic cDNA in vaccinia virus. We show that the recombinant virus gen...

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Autors principals: Coley, Scott E., Lavi, Ehud, Sawicki, Stanley G., Fu, Li, Schelle, Barbara, Karl, Nadja, Siddell, Stuart G., Thiel, Volker
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2005
Matèries:
Pathogenesis and Immunity
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC548458/
https://ncbi.nlm.nih.gov/pubmed/15709029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.79.5.3097-3106.2005
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