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Inference of relationships in the ‘twilight zone’ of homology using a combination of bioinformatics and site-directed mutagenesis: a case study of restriction endonucleases Bsp6I and PvuII

Thus far, identification of functionally important residues in Type II restriction endonucleases (REases) has been difficult using conventional methods. Even though known REase structures share a fold and marginally recognizable active site, the overall sequence similarities are statistically insign...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Pawlak, Sebastian D., Radlinska, Monika, Chmiel, Agnieszka A., Bujnicki, Janusz M., Skowronek, Krzysztof J.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2005
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC548357/
https://ncbi.nlm.nih.gov/pubmed/15684412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gki213
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