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Purification of a 110-kilodalton cytosolic phospholipase A2 from the human monocytic cell line U937.

The major dithiothreitol-resistant phospholipase A2 activity present in the cytosol of U937 cells has been purified greater than 200,000-fold by sequential chromatography on phenyl-5PW, heparin-Sepharose CL-6B, high-performance hydroxylapatite, TSK-gel G3000-SW, and Mono Q columns. This 110-kDa cyto...

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Main Authors: Clark, J D, Milona, N, Knopf, J L
Format: Artigo
Jezik:Inglês
Izdano: 1990
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC54817/
https://ncbi.nlm.nih.gov/pubmed/2217203
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