Duplex Reverse Transcription-PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses

A new approach was developed for the rapid detection and identification of Brazilian alphaviruses and flaviviruses. The methodology involves the genus-specific detection of Alphavirus and Flavivirus by a duplex reverse transcription-PCR (D-RT-PCR), followed by multiplex nested PCR (M-N-PCR) or neste...

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Detalhes bibliográficos
Main Authors: Bronzoni, Roberta Vieira de Morais, Baleotti, Flávia Graciela, Ribeiro Nogueira, Rita Maria, Nunes, Márcio, Moraes Figueiredo, Luiz Tadeu
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2005
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC548032/
https://ncbi.nlm.nih.gov/pubmed/15695666
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.43.2.696-702.2005
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