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Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

Successful establishment of CRISPR/Cas9 genome editing technology in Plasmodium spp. has provided a powerful tool to transform Plasmodium falciparum into a genetically more tractable organism. Conditional gene regulation approaches are required to study the function of gene products critical for gro...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Knuepfer, Ellen, Napiorkowska, Marta, van Ooij, Christiaan, Holder, Anthony A.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5478596/
https://ncbi.nlm.nih.gov/pubmed/28634346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03984-3
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