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Generating conditional gene knockouts in Plasmodium – a toolkit to produce stable DiCre recombinase-expressing parasite lines using CRISPR/Cas9

Successful establishment of CRISPR/Cas9 genome editing technology in Plasmodium spp. has provided a powerful tool to transform Plasmodium falciparum into a genetically more tractable organism. Conditional gene regulation approaches are required to study the function of gene products critical for gro...

詳細記述

保存先:
書誌詳細
出版年:Sci Rep
主要な著者: Knuepfer, Ellen, Napiorkowska, Marta, van Ooij, Christiaan, Holder, Anthony A.
フォーマット: Artigo
言語:Inglês
出版事項: Nature Publishing Group UK 2017
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5478596/
https://ncbi.nlm.nih.gov/pubmed/28634346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03984-3
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