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Combining optogenetics and electrophysiology to analyze projection neuron circuits

A set of methods is described for channelrhodopsin-2 (ChR2) based synaptic circuit analysis that combines photostimulation of virally transfected presynaptic neurons’ axons with whole-cell electrophysiological recordings from retrogradely labeled postsynaptic neurons. The approach exploits the prese...

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Detalhes bibliográficos
Publicado no:Cold Spring Harb Protoc
Main Authors: Yamawaki, Naoki, Suter, Benjamin A., Wickersham, Ian R., Shepherd, Gordon M. G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5476926/
https://ncbi.nlm.nih.gov/pubmed/27698240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot090084
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