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Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca(++) sensing

Optogenetic techniques allow intracellular manipulation of Ca(++) by illumination of light-absorbing probe molecules such as channelrhodopsins and melanopsins. The consequences of optogenetic stimulation would optimally be recorded by non-invasive optical methods. However, most current optical metho...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nat Commun
Hauptverfasser: Yang, Jie, Cumberbatch, Derrick, Centanni, Samuel, Shi, Shu-qun, Winder, Danny, Webb, Donna, Johnson, Carl Hirschie
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5476805/
https://ncbi.nlm.nih.gov/pubmed/27786307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms13268
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