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Reduction of DNA Contamination in RNA Samples for RT-PCR using Selective Precipitation by Compaction Agents
An important problem in measurement of mRNA levels by RT-PCR is DNA contamination, which can produce artifactually increased mRNA concentration. Current methods to eliminate contaminating DNA can compromise the integrity of the RNA, are time-consuming, or are hazardous. We present a rapid, nuclease-...
Gardado en:
| Publicado en: | Anal Biochem |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5476456/ https://ncbi.nlm.nih.gov/pubmed/18831957 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.09.009 |
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