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Prenylated proteins: synthesis of geranylgeranylcysteine and identification of this thioether amino acid as a component of proteins in CHO cells.

Prenylated proteins, labeled in the isoprenoid residue by growing CHO cells in medium containing [5-3H]mevalonate, were degraded by three different proteolytic procedures, enzymatic or alkaline hydrolysis as well as hydrazinolysis. The products thus obtained were analyzed by HPLC with chemically pre...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Epstein, W W, Lever, D C, Rilling, H C
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1990
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Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC54744/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2217168/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.87.19.7352
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